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Authors
Source
Indian Forester, Vol 137, No 9 (2011), Pagination: 1066-1070
Abstract
A total of 250 random oligonucleotide primers were surveyed to identify sex of Simarouba glauca a tree born oilseed crop. Primer OPA-OS900, was found to produce a consistent marker, which differentiated female from both male and andromonoecious sex plants. An identified random amplified polymorphic DNA (RAPD) marker, OPA-OS, linked to male sex in Simarouba was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region (SCAR) primer SCAR(12f/r), which was found promising in male and andromonoecious sex. The marker designed was amplified at a specific site of 915 bp only in male and andromonoecious plants. This would help to identify the sex of tree born oilseed crop Simarouba.
Keywords
Simarouba glauca, Sex Detection, Molecular Marker, RAPD SCAR Marker
